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Real time PCR

The Real-Time CFX OPUS 96 well (Bio-Rad) is a new generation instrument for the analysis of gene expression, Copy Number Variation (CNV) and High Resolution Melting (HRM).

  • Compatible with all reagents and chemicals on the market
  • Possibility to analyze up to 5 targets in the same reaction (Multiplex)
  • Can be used without the use of a PC ('stand alone mode')
  • Block for 96 well plates
  • Possibility to access the BR.io 'cloud' to set up protocols and data analysis
  • No need for additional dye as a passive fluorophore

Other:

"CFX Maestro 2.0" data analysis software

  • Statistical analysis of gene expression with t-test and ANOVA
  • Stability analysis of reference genes (housekeeping genes) and direct selection of the most stable using the GeNorm algorithm
  • Possibility to analyze hundreds of plates using the ANOVA statistical analysis on all collected data by means of the "Gene Study" function 
  • "Precision Melt Analysis" interpretation software for HRM
  • Email notification at the end of the run with sending of report and results
  • High resolution image graphics suitable for scientific publications or presentations

  • Gene expression evaluation
  • Copy Number Variation (CNV) analysis

intermediate (requires initial training with expert staff).

  • Experimental design consultancy;
  • training of new users;
  • technical assistance;
  • the service is also open to direct collaborations on specific projects.

Access instructions for UniTo users:

  1. Read the Rule Book carefully.
  2. For each instrument for which access is requested:
    a) Fill in the specific “Access request” - UniTo users;
    b) Send to openlab.dsv@unito.it the Annex A present in the Rule Book, signed by the Operator and PI.
    Step a) and b) to be carried out simultaneously.
    Failure to receive the Annex A will make it impossible to book and use the required equipment.
  3. Only after receiving confirmation email from Open Lab staff (all sent data and documentations have to be verified):
    - For all DSV users and non-DSV users with a subscription: book the instrument from the Open Lab page through CampusNet platform with your SCU credentials.
    - For non-DSV user with a single use rate: book the instrument through the email openlab.dsv@unito.it.

 

Access instructions for NON-UniTo users:

  1. Read the Rule Book carefully.
  2. For each instrument for which access is requested:
    a) Fill in the specific "Access request" - NON-UniTo users;
    b) Send to openlab.dsv@unito.it the Annex B present in the Rule Book, signed by the Operator and PI, and document "Legge 136" (english version).
    Step a) and b) to be carried out simultaneously.
    Failure to receive the Annex B and the document "Legge 136" will make it impossible to book and use the required equipment.
  3. Only after receiving confirmation email from Open Lab staff (all sent data and documentations have to be verified):
    Book the instrument through the email openlab.dsv@unito.it.

 

 
Last update: 13/06/2024 16:39
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