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Droplet Digital PCR

The Platform for DROPLET DIGITAL PCR QX-200 Biorad represents an innovative technology obtained from the evolution of quantitative PCR or Real time PCR. It allows to quantify nucleic acids in an absolute way without the need for calibrators or reference curves. It is particularly useful for low abundance targets, complex background targets, allelic variants (SNPs), and for monitoring changes in the expression levels of genes that cannot be detected with real time PCR.

 

The platform includes:

  • QX200 Droplet Generator
  • C1000 Touch Thermal Cycler
  • PX1 PCR Plate Sealer
  • QX200 Droplet Reader

  • Microbiology: identification of pathogens (viruses, bacteria and genetically modified microorganisms), quantification of the viral load, study of the microbiome
  • AMR: detection and monitoring of antibiotic resistance
  • NGS: amplification and quantification of libraries, validation of sequencing results
  • Oncology: Copy number variation (CNV) analysis, identification of rare alleles, quantification of mutations also in nucleic acids extracted from FFPE tissues, copies of rare target-DNA, liquid biopsies
  • Gene Expression Analysis: reliable and reproducible measurements of small expression changes even of low abundance mRNA and microRNA

intermediate (requires initial training with experienced staff).

  • Experimental design consultancy;
  • training of new users;
  • technical assistance;
  • the service is also open to direct collaborations on specific projects.

Access instructions for UniTo users:

  1. Read the Rule Book carefully.
  2. For each instrument for which access is requested:
    a) Fill in the specific “Access request” - UniTo users;
    b) Send to openlab.dsv@unito.it the Annex A present in the Rule Book, signed by the Operator and PI.
    Step a) and b) to be carried out simultaneously.
    Failure to receive the Annex A will make it impossible to book and use the required equipment.
  3. Only after receiving confirmation email from Open Lab staff (all sent data and documentations have to be verified):
    - For all DSV users and non-DSV users with a subscription: book the instrument from the Open Lab page through CampusNet platform with your SCU credentials.
    - For non-DSV user with a single use rate: book the instrument through the email openlab.dsv@unito.it.

 

Access instructions for NON-UniTo users:

  1. Read the Rule Book carefully.
  2. For each instrument for which access is requested:
    a) Fill in the specific "Access request" - NON-UniTo users;
    b) Send to openlab.dsv@unito.it the Annex B present in the Rule Book, signed by the Operator and PI, and document "Legge 136" (english version).
    Step a) and b) to be carried out simultaneously.
    Failure to receive the Annex B and the document "Legge 136" will make it impossible to book and use the required equipment.
  3. Only after receiving confirmation email from Open Lab staff (all sent data and documentations have to be verified):
    Book the instrument through the email openlab.dsv@unito.it.

 

Last update: 13/06/2024 16:37
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